دكتر هوشنگ رفعت پناه (MD, PhD ایمونولوژی)
عضو هیئت علمی گروه ایمونولوژی
آدرس: مشهد، ميدان آزادي، ساختمان پرديس دانشگاه، دانشکده پزشکي، بخش ايمونولوژي
تلفن: 05118002376 و 05118012762
1999-2003 PhD in Immunology
·Department of Immunology, The University of Manchester
·Title: Immunogenetic analysisof patients with HTLV-I (human T lyphotropic virus type I) infection: associations with HLA, cytokine and Perforin gene polymorphisms
·Project: Investigation the hypothesis that genetic factors may account for the differences in the outcome of HTLV-I infection, namely HTLV-I carrier status or HTLV-associated myelopathy/tropical spastic paralysis (HAM/TS).
1992-1994 MSc in Immunology
·Dept of Immunology, Mash-had University of Medical Sciences, Mash- had, Iran
·Dissertation project: Association between HLA-DR and insulin dependent diabetes type
1983-1987 BSc. in laboratory sciences
Iran University of Medical Sciences, Tehran, Iran.
2003-Present Assistant professor of Immunology
·Dept of Immunology, Ghaem Hospital, Mash-had University of Medical Sciences, Mash-had-Iran.
· Teaching Immunology to undergraduate and postgraduate students.
·I supervised three Master students, and two fellowship theses. In each case this involved assisting in project management, development and completion.
·I was awarded three grants from the chancellor of research, Mash-had University of Medical Sciences. The aim of these projects was to understand the role of Immunogenetic factors in human T lymphotropic virus type I (HTLV-I) and hepatitis B virus.
·Several publications resulted from these work.
·Head of HTLV-I Research lab,
1994-1998 Lecturer of Immunology
· Dept of Immunology, Ghaem Hospital Mash-had university of Medical Sciences, Mash-had, Iran.
Ghaem Hospital, Mash-had University of Medical Sciences, Mash-had, Iran.
1987-1992 Teaching assistant
· Dept of Immunology, Ghaem Hospital, Mash-had University of Medical Sciences, Mashhad-Iran.
·Human Tlymphotropic virus type (HTLV-I) is associated with HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/TSP) and adult T-cell leukaemia (ATL). Fewer than 2% of HTLV-I-infected individuals develop HAM/TSP. The different outcome of HTLV-I infection may be explained by the existence of viral agents, genetic background or even environmental factors. My research aim was to study the correlation between n cytokine gene polymorphisms, mainly Th1 and Th2, in Iranian patients with HAM/TSP, asymptomatic HTLV-I carriers and healthy controls. We also analysed the influence of HLA class II, RB1 alleles and class I HLA-A2, A30, Cw8 and B54 alleles in all three groups. I screened the promoter and first intron of the perforin gene, as a key factor in elimination of viral-infected cells, to determine novel polymorphisms. A novel polymorphism at position +418 relative to the transcription start site was detected. My research output from my PhD studies are summarized in the publication section (please see below).
I mainly investigated the role of cytokines gene polymorphism, HLA and pefrorin in HTLV-I infection individuals. My PhD research involved the following laboratory techniques: flow cytometry/immuno-fluorescent staining, cell culture, cell sorting using micro-beads, PCR, SSCP, RT-PCR, cDNS synthesis and sequencing, SNP genotyping techniques, SDS-page and agarose gel electrophoresis, western blotting, and the use of radioactive isotopes (gamma radiation), DNA cloning, cell line transfection, protein assay and gel shift assay.
·I have knowledge of all Microsoft office programs and whilst at Manchester university attended advanced courses in Excel, PowerPoint and Word. I have a good knowledge in Bioinformatics and experienced in the use of different web sites in this field. I am familiar in a range of statistic software packages including SPSS and STATA.
·Rafatpanah H, Pravica V, Farid R, Tabatabaei A, Ollier A, Poulton K, Thomson W, Hutchnison IV. Association between
HLA-DRB*1 and HLA-Cw*08 and outcome following HTTLV-I infection (submitted to International Journal of Immunogenetics).
· Rafatpanah H, Pravica V, Farid R, Abbaszadegan MR, Tabatabaei A, Goharjoo A, Hutchinson IV.
Association of IL-10 gene polymorphisms and HTLV-I infection in northeast Iran (Mash-had) (submitted).
·Reza Farid, Ramin Ghasemi, Mahmood Baradaran-Rahimi, Farahzad Jabbari, Javad Ghaffari, and Houshang Rafatpanah.
Evaluation of Six Years Allergen Immunotherapy in Allergic Rhinitis and Allergic Asthma. IJAAI, 2006; 5: 29-31.
·Faridhosseni R.., Ghaffari J., Rafatpanah H., Jabbary F., Taghavi M., Shakery M.T., Thyroid Auto antibodies in Chronic Urticaria.Medical Journal of MUMS, 2006; 48:373-378 (Persian).
·Farid R, Ghafari J, Parizadeh M, Miri S, Rafatpanah H. Prevalence of HTLV-I infection in Neishabour-Iran.
Medical Journal of MUMS 2005; 47:417-424 (Persian).
·R. Farid , Shirdel , M. Etemadi , H. Rafatpanah, H. Baradaran , F. Farid, B. Nikbin Phylogenetic of Human T-cell
Lymphotropic Virus Type I in Iranians Born in Mashhad. AIM 1999; 2:20-24.
·Iranian Society of Allergy and Immunology
·British Society of Immunology (BSI)